BBL Pouch Systems - EZ Pouch Systems -
The older style BBL Pouch systems have been replaced by the newer style
EZ Pouch systems listed below. To ease the conversion to the newer EZ pouch,
both the older BBL Pouch and the replacement EZ Pouch is listed
below.
PRINCIPLES OF THE PROCEDURE ~ The
GasPak™ EZ Gas Generating Pouch Systems are single-use systems that
produce atmospheres suitable to support the primary isolation and cultivation
of anaerobic, microaerophilic, or capnophilic bacteria by use of gas generating
sachets inside single-use resealable pouches. The GasPak EZ Gas Generating
Sachet consists of a reagent sachet containing inorganic carbonate, activated
carbon, ascorbic acid and water. When the sachet is removed from the outer
wrapper, the sachet becomes activated by exposure to air. The activated reagent
sachet and specimens are placed in the GasPak EZ Incubation Container and the
container is sealed. The sachet rapidly reduces the oxygen concentration within
the container. At the same time, inorganic carbonate produces carbon dioxide.
**For the cultivation of anaerobic bacteria, the
GasPak EZ Anaerobe Container System Sachets produce an anaerobic atmosphere
within 2.5 h, with greater than 15% carbon dioxide within 24 h.
**For the cultivation of microaerophilic bacteria, the GasPak
EZ Campy Container System Sachets produce a microaerophilic atmosphere within 2
h with approximately 6-16% oxygen, and 2-10% carbon dioxide within 24 h.
**For the cultivation of carbon dioxide requiring
organisms, the GasPak EZ CO2 Container System Sachets produce an atmosphere
within 24 h with greater than or equal to 2.5% carbon dioxide. |
